Quaternary ammonium chlorides [August 24, 2004]
Posted: Sun Aug 29, 2004 8:37 pm
Chromatography Forum: LC Message Board: Quaternary ammonium chlorides
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By isabel on Tuesday, August 24, 2004 - 06:04 am:
I see a message that there is a simple chromatographic method with a solution hexane sulfonic acid for analysing quaternary ammonium chlorides!
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By SIELC_Tech on Tuesday, August 24, 2004 - 06:36 am:
Dear Isabel,
Please check the following links for the analysis of quaternary amines. You do not have to use ion-pairing reagent (hexane sulfonic acid)with our Primesep columns. Two main problems with quaternary amines is lack of retention on RP column and poor peak shape.
http://allsep.com/makeChr.php?chr=Chr_047
http://allsep.com/makeChr.php?chr=Chr_043
http://allsep.com/makeChr.php?chr=Chr_007
Here is the comparisson for regular RP and Primesep column:
http://allsep.com/makeCmp.php?cmp=Cmp_080
Please contact us if you need help in developing a new method.
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By isabel on Tuesday, August 24, 2004 - 06:43 am:
Thank's a lot but i want a specify uv method for chloromequat choline chloride and choline chloride
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By SIELC_Tech on Tuesday, August 24, 2004 - 07:50 am:
Cholines in general are not UV acive (accept derivatives like acetyl choline, carbachol, etc.). You either have to do derivatization or use inderect detection. Other approaches include LC/MS and ELSD detection techniques.
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By A.N. Onimis on Wednesday, August 25, 2004 - 07:35 pm:
Go here: http://www2.waters.com/watprod.nsf/Newdocs/WA40482
You may need to register.
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By Chris Pohl on Thursday, August 26, 2004 - 06:27 pm:
Compounds such as choline and acetyl choline are easily detected without derivatization by making use of conductivity detection http://www1.dionex.com/en-us/webdocs/ap ... 70_V15.pdf
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By isabel on Tuesday, August 24, 2004 - 06:04 am:
I see a message that there is a simple chromatographic method with a solution hexane sulfonic acid for analysing quaternary ammonium chlorides!
-------------------------------------------------------------------------------------------------------
By SIELC_Tech on Tuesday, August 24, 2004 - 06:36 am:
Dear Isabel,
Please check the following links for the analysis of quaternary amines. You do not have to use ion-pairing reagent (hexane sulfonic acid)with our Primesep columns. Two main problems with quaternary amines is lack of retention on RP column and poor peak shape.
http://allsep.com/makeChr.php?chr=Chr_047
http://allsep.com/makeChr.php?chr=Chr_043
http://allsep.com/makeChr.php?chr=Chr_007
Here is the comparisson for regular RP and Primesep column:
http://allsep.com/makeCmp.php?cmp=Cmp_080
Please contact us if you need help in developing a new method.
-------------------------------------------------------------------------------------------------------
By isabel on Tuesday, August 24, 2004 - 06:43 am:
Thank's a lot but i want a specify uv method for chloromequat choline chloride and choline chloride
-------------------------------------------------------------------------------------------------------
By SIELC_Tech on Tuesday, August 24, 2004 - 07:50 am:
Cholines in general are not UV acive (accept derivatives like acetyl choline, carbachol, etc.). You either have to do derivatization or use inderect detection. Other approaches include LC/MS and ELSD detection techniques.
-------------------------------------------------------------------------------------------------------
By A.N. Onimis on Wednesday, August 25, 2004 - 07:35 pm:
Go here: http://www2.waters.com/watprod.nsf/Newdocs/WA40482
You may need to register.
-------------------------------------------------------------------------------------------------------
By Chris Pohl on Thursday, August 26, 2004 - 06:27 pm:
Compounds such as choline and acetyl choline are easily detected without derivatization by making use of conductivity detection http://www1.dionex.com/en-us/webdocs/ap ... 70_V15.pdf