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Nucleotides triphosphate separation on HPLC

Posted: Thu Sep 07, 2006 2:32 pm
by Ronny
Hi All,

I want to separate nucleotides triphosphate (rNTP) by HPLC but I don't know what column to use, maybe you can help me.

Thanks,

Ronny

Posted: Thu Sep 07, 2006 3:53 pm
by Bryan Evans
We have ATP separations on both our
Cadenza CD-C18 column and our Unison UK-C18 columns.


http://www.imtakt.com/TecInfo/TI032E.pdf
http://www.imtakt.com/TecInfo/TI133E.pdf

Posted: Fri Sep 08, 2006 9:23 am
by Tobias Jonsson
Instead of using C18 and ion-pair reagents, HILIC is a good option for these kind of separations.

http://www.sequant.com/sn/ufiles/SeQuan ... 00-03A.pdf