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Protocol for Lactose Separation using Chromatography
Posted: Wed Sep 06, 2006 2:00 am
by smsaufi
Hi,
I try to separate lactose using chromatography system. Does anyone can help me to get the protocol for that?
Thanks in advance.
Saufi
Posted: Wed Sep 06, 2006 2:11 am
by Uwe Neue
Separate from what?
Here is a possible method: Waters Carbohydrate Analysis column with 75% acetonitrile and 25% water.
Posted: Wed Sep 06, 2006 4:28 am
by smsaufi
Actually I used standard lactose in water. I try to develop new affinity ligand that can bind a lactose. I plan to check the static and dynamic binding capacity for my new ligand. But, I'm not very sure about the suitable mobile phase for that binding experiment.
Posted: Wed Sep 06, 2006 7:44 pm
by Uwe Neue
The chromatographic system that I proposed can be used for assaying the lactose concentration.
I can not advise you how to do the binding to your affinity ligand, since I do not know what it is.
Posted: Thu Sep 07, 2006 12:28 am
by smsaufi
The ligand used is peptide. Anyway thanks for your help:)
Posted: Thu Sep 07, 2006 6:46 pm
by tom jupille
If all you need to see is the single peak, there are numerous carbohydrate analysis columns available, designed to run with different mobile phases:
- water/ACN (per Uwe's suggestion),
- pure water (e.g., HPX-87 series columns from Bio-Rad), or
- basic buffers (e.g., columns designed for use with the PAD detector from Dionex).