THF in mobile phase [August 20, 2004]
Posted: Sun Aug 29, 2004 8:28 pm
By ylyuen on Friday, August 20, 2004 - 02:43 am:
Hi!
I have a method which mobile phase is 20% THF. O doubt that the THF can actually harm the column cause the column can easily faced high pressure problem if it does not flush 24 hours with distilled water.
TQ!
yenle
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By a on Friday, August 20, 2004 - 05:26 am:
THF can be used on RP columns without too many problems. Be aware that some THF is stabilized with BHT, which will retain and eventually elute off the column.
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By Consumer Products Guy on Friday, August 20, 2004 - 07:45 am:
We use THF routinely with RP columns, when necessary to produce better separations, no issues. We use non-stabilized THF.
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By S. Girault on Tuesday, August 24, 2004 - 09:37 am:
be carefull, the major problem may be the peek parts of your system (visible and unvisible injection loop, detector flow cell)which wont apreciate this so high level of THF.
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By Anonymous on Tuesday, August 24, 2004 - 10:44 am:
In addition to the detector flow cell, the autosampler injection stator is commonly made of polymeric material (e.g., Tefzel) that is prone to polymeric swelling w/THF. PEEK tubing and fittings can also be susceptible.
Hi!
I have a method which mobile phase is 20% THF. O doubt that the THF can actually harm the column cause the column can easily faced high pressure problem if it does not flush 24 hours with distilled water.
TQ!
yenle
-------------------------------------------------------------------------------------------------------
By a on Friday, August 20, 2004 - 05:26 am:
THF can be used on RP columns without too many problems. Be aware that some THF is stabilized with BHT, which will retain and eventually elute off the column.
-------------------------------------------------------------------------------------------------------
By Consumer Products Guy on Friday, August 20, 2004 - 07:45 am:
We use THF routinely with RP columns, when necessary to produce better separations, no issues. We use non-stabilized THF.
-------------------------------------------------------------------------------------------------------
By S. Girault on Tuesday, August 24, 2004 - 09:37 am:
be carefull, the major problem may be the peek parts of your system (visible and unvisible injection loop, detector flow cell)which wont apreciate this so high level of THF.
-------------------------------------------------------------------------------------------------------
By Anonymous on Tuesday, August 24, 2004 - 10:44 am:
In addition to the detector flow cell, the autosampler injection stator is commonly made of polymeric material (e.g., Tefzel) that is prone to polymeric swelling w/THF. PEEK tubing and fittings can also be susceptible.