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Agilent lcmsd successive SIM monitoring w/o sensitivity loss

Posted: Mon May 29, 2017 11:29 pm
by mretier
Hi,
I am monitoring three ions which elute at different time points, an internal standard and two ions of interest. In Chemstation, I setup a method which starts monitoring ion 1 from time 0, from time 4 it monitors ion 2, from time 8 it monitors ion 3.
The method works, however, compared to a method that only monitors one of the ions of interest the sensitivity is significantly reduced.

Is there a reason for this behavior?

Thanks,
M

Re: Agilent lcmsd successive SIM monitoring w/o sensitivity loss

Posted: Tue May 30, 2017 9:13 am
by Rndirk
No, it should have the same sensitivity.

If you want us to find the problem, i think the easiest way would be to post screenshots of the MS settings in both methods.