How stable are Primesep columns at higher pH, say 6-6.5?

[fatBrain]

I am revising an impurity test method for carboxylic acids using HPLC/UV. GC method works just fine for API, however, there are too many placebo peaks for drug products by using the same GC method. To get rid of these peaks, relatively complicated wet chem procedures may be needed.

My thinking is that it is possible to push excipient peaks to early front using Primesep B/B2. Only problem could be, how long can Primesep B/B2 last at this pH? The vendor recommends a pH from 1.5 to 4.5 for Primesep B. But at this pH, carboxylic acids may not ionize. Or, may Primesep D be a good alternative?

Thanks in advance for all replies.


Fatbrain

[SIELC_Tech]

FatBrain,

Please provide me with your email and phone number and we will discuss your separation.

http://www.sielc.com/AboutUs_ContactUs.html

May be after you describe your system we can help you with the method. In addition to that we can develop a method for you-Free of Charge. We have few other columns which might work for you.

Our office is in Illinois, USA and alternatively you can call us any time.

Kind regards,

Vlad

[fatBrain]

Thanks, Vlad.

My phone number is 510-476-2000, ext 1171, and my email address is jasonguo@impaxlabs. com. Since I am often away from my desk, email is a better means to discuss.

By the way, I have already placed an order for a Primesep B2 column to try out last Friday. Additional help from you will be greatly appreciated. Looking forward to discussing with you!

[HW Mueller]

Vlad, you know that some of us feel sadly left out when someone answers public questions in private.....