Chromatography Forum

Just curious what do your think about the most challenging/most difficult part facing the LC/MS analysis? How do you usually deal with it? Thanks.

Depends on what you are doing...

If it is analyses from a biological matrix, I would think that ion-suppresion is the general problem.

Uwe, Thanks for your answer. What do you do to minimize the ion suppression effect? What else beside ion suppression? Thanks so much.

To reduce ion suppression stemming from a complex matrix such as biofluids, you need to reduce the complexity of the sample with solid sample clean-up techniques such as SPE and high-quality chromatographic separations.

You can test for ion-suppression issues by doing post--column infusion of the analyte(s).