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sunfire and aflatoxins

http://www.chromforum.org/viewtopic.php?t=4417

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sunfire and aflatoxins

Posted: Thu Aug 10, 2006 12:05 pm
by n-cute
Hi,
I'm preparing to aflatoxins analysis and have to choose between few kind of Waters RP columns: Spherisorb S3ODS2 (3um, 80A, 4,6x150 mm), Spherisorb S5ODS2 (5um, 80 A, 4,6x250mm) and Sunfire C18 (3,5um, 100 A, 4,6x150 mm). does anyone of You have practice with that kind of columns and could suggest me "right" choice? I think, what SunFire can do the work, but I'm not sure. :?

Posted: Thu Aug 10, 2006 3:58 pm
by Uwe Neue
Modern columns give better peak shapes for all problematic compounds and tend to be more reproducible. I would go with the SunFire column...

Posted: Thu Aug 10, 2006 4:34 pm
by Mark Tracy
The first time I did aflatoxins, back in the 1980's, I used a Spherisorb ODS2. It worked fine. Since then I have used various C18 columns, and they all work fine. Aflatoxins are well behaved chromatographically. You can decide on price or on column reproducibility; either way you will get acceptable chromatography.

:)

Posted: Fri Aug 11, 2006 6:31 am
by n-cute
i have decided to SunFire. thx for Your advices:)
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