HPLC - Empower

[flower25]

Hi,

I recently started to use Empower 3 instead of Chemstation. In Component Editor, in Empower, there is a tab called "multiplier". I never encountered that in Chemstation. What exactly is it? and how can I manually calculate it? Does anybody know how (formula) that Empower uses to calculate %Assay using the sample weight in sample set, value (amount that we enter in Component Editor table), LC, and multiplier?

Thanks.

[shaun78]

Never use the multiplier.... never ever.

It will multiply your raw data by a value of your choosing; and raw data is inclusive of datapoint collected by your detector.

As for calculating % Assay, Empower uses common linear regression / least squares calculations by default. If you are running a single point, then the software simply forces a zero origin to obtain a best fit line.