Chromatography Forum

Hello,

I am trying to analyse beerepellents
(Benzaldehyde, Phenol, p-Dichlorobenzene, Nitrobenzene, p-Bromochlorobenzene(ISTD), Naphthalene, Thymol)
in honey. Which column can I use? I tried HP-5MS column, but I couldn't have good peaks for especially Phenol.

Best regards,

For phenol analysis I used (in a past life) an amino column.

A wax phase should do you nicely for the phenol as well as the compounds with all of the benzene rings.

Review this article. The other compounds fluoresce as well.

https://www.ncbi.nlm.nih.gov/pubmed/12926862

Hello,

I am trying to analyse beerepellents
(Benzaldehyde, Phenol, p-Dichlorobenzene, Nitrobenzene, p-Bromochlorobenzene(ISTD), Naphthalene, Thymol)
in honey. Which column can I use? I tried HP-5MS column, but I couldn't have good peaks for especially Phenol.

Best regards,

We analyze Phenol using a Rxi-5SilMS column. You have to begin the oven program at 35-40C and have at least a 1 minute initial hold. You have to begin the data acquisition early, just in the tail of the solvent peak, if using dichloromethane. It is possible. The Phenol will have some tailing but it is still nice and linear in calibration.

Hello,

I am trying to analyse beerepellents
(Benzaldehyde, Phenol, p-Dichlorobenzene, Nitrobenzene, p-Bromochlorobenzene(ISTD), Naphthalene, Thymol)
in honey. Which column can I use? I tried HP-5MS column, but I couldn't have good peaks for especially Phenol.

Best regards,

It is quite likely that your column and/or inlet have developed active sites that make the peaks tail. A new column of the same phase or a PEG wax phase, and a clean inlet liner will probably solve the problem.

Peter

Thank you all for the replies.