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degasser
Posted: Sun Jul 30, 2006 7:25 am
by sudhis
hi everybody ,
as we knew that there is a degasser for the degassing of mobile phase in HPLC , i would like to know what is the input of a degasser and the mechanism of it.
regards,
sudhi
Posted: Mon Jul 31, 2006 12:25 am
by Kostas Petritis
Most degasseurs uses vaccum along with micro-porous tubing...
Posted: Mon Jul 31, 2006 5:44 pm
by Mark Tracy
The degasser passes the mobile phase through gas-permeable fluorocarbon tubing. The tubing is inside a vacuum chamber. The original designs (~1992) used macro-porous PTFE, and were susceptible to contamination, had a large internal volume (15 mL). With time, better tubing materials (Teflon AF) and vacuum control have reduced the volume (2mL) and resistance to contamination (no macro-pores). The exhaust from the vacuum contains the gasses removed from the mobile phase, plus whatever solvent can diffuse through the teflon.