mV settings in electrchemical detection system.

[paraboxa]

Can anyone tell me how to select mV settings for electrochemical detection? I'm working on a methyl transferase with a C18 column, isocratic system. Mobile phase is phosphate-acetonitrile-THF at pH6.2. Samples are patient EDTA blood.

[tom jupille]

What, exactly, are you trying to detect? And what, exactly, detector are you using (manufacturer, and model number)?

[Markus Laeubli, Metrohm]

The mV setting (working potential) defines your detector selectivity. An optimized setting may discriminate interfering peaks.
The working potential depends on the component to be analyzed.