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HPLC-RID for sugar (50 ppm)
Posted: Fri Apr 14, 2017 3:37 am
by waluyo
Hi,
I'm planning to analyze sugar (glucose, fructose) with the lowest concentration of 50 ppm. My instrument will be HPLC Agilent 1100 (with RID 1100 series also).
Is it possible to analyze sugar with RID for concentration of 50 ppm?
What is the possible column or mobile phase?
Thanks
Re: HPLC-RID for sugar (50 ppm)
Posted: Fri Apr 14, 2017 7:41 am
by Rndirk
Hi,
Yes, it will be possible. You can find plenty of methods if you search for "HPLC-RI sugars".
Re: HPLC-RID for sugar (50 ppm)
Posted: Fri Apr 14, 2017 11:12 am
by HPLC chemist
An RID is too insensitive. The best concentration I could detect is 1000 ppm or 0.1% (30 years ago). Your better of to use a Biorad Aminex column (or equivalent) and 210 nm UV.
Check AOAC or ICUMSA (International Commission for Methods of Sugar Analysis) for an appropriate method based on your sample matrix and expected concentration.
Re: HPLC-RID for sugar (50 ppm)
Posted: Fri Apr 14, 2017 1:22 pm
by Consumer Products Guy
Got ELSD ?
Re: HPLC-RID for sugar (50 ppm)
Posted: Wed Apr 19, 2017 3:49 pm
by James_Ball
We have also been looking into methods for Sugars and Dietary Fiber and they both use RID. I am wondering if LC/MS would be a good option or would there be problems ionizing the sugars?