HCG

[upamaniah]

Hi,
i would like to analyse HCG harmone by hplc for assay, any tips

[HPLC chemist]

You mean this;

https://www.researchgate.net/publicatio ... S_Analysis

[upamaniah]

Hi,
no really similar, i would like to analyse using UV for assay without denaturing

[HPLC chemist]

If it is a drug product try 214 nm where the peptide bond absorbs. If it is a blood/plasma sample you're stuck with LC/MS/MS.

It ALL depends on the expected concentration (ppm versus ppt) and the sample matrix!

[HPLC chemist]

Column, mobile phase, and other chromatographic conditions remain the same.