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[pliva]

dear friends,
i was working for adl formulation pharmaceutical research lab but now i am in bio analytical lab where we are concerned about BA and BE studies. as it is new to me could you pliz help me regarding following queries.

1) from which website can i get the reference for bioanalytical methods like as i go through USP,BP etc.
2) which website provide pharmacokinetic details of the drug like c max t max and also probable metabolite.
3) is uplc is prefered than hplc as sample size is very very less in bio analytical.
4) use of internal standard is compulsory in bio analytical methods?

with regard
pliva

[pliva]

dear friends,
i was working for adl formulation pharmaceutical research lab but now i am in bio analytical lab where we are concerned about BA and BE studies. as it is new to me could you pliz help me regarding following queries.

1) from which website can i get the reference for bioanalytical methods like as i go through USP,BP etc.
2) which website provide pharmacokinetic details of the drug like c max t max and also probable metabolite.
3) is uplc is prefered than hplc as sample size is very very less in bio analytical.
4) use of internal standard is compulsory in bio analytical methods?
i am still waiting for your reply.pliz

with regard
pliva

[Uwe Neue]

1. We have developed generic methods for the extraction of the analytes from biological matrices such as plasma and urine. These methods can be fine-tuned, if needed. Look at the Waters website for applications of the Oasis family of packings.
3. UPLC is preferred, but the sensitivity advantages are small compared to a well-thought-out HPLC set-up.
4. There is no way around using an internal standard, and even with this there are problems due to ion suppression. There was an article in Anal. Chem. by Matushewski that describes ways to get around the problems posed by biological matrices. I do not have the reference handy, but you should be able to find it readily and I highly recommend to read this article.

I found it: Matuszewksi, Strategies for the assessment of matrix effects in quantitative bioanalytical methods ... Anal Chem 75 (2003), 3019

[sandip.gurav]

Hi

Following web sites could help in Referencing

www.rxlist.com,www.pubmed.com,www.sciencedirec.com

[james little]

On matrix effects, see the first reference on my web site.

Really good book.

My paper also talks about matrix effects of phospholipids in particular..

http://littledomain.com/james/files/text.pdf

[pliva]

On matrix effects, see the first reference on my web site.

Really good book.

My paper also talks about matrix effects of phospholipids in particular..

http://littledomain.com/james/files/text.pdf

thank you james and thank you sandip. thanks all for your valuable feed back.