Advertisement
Chromatography Forum

Column wash

Discussions about GC-MS, LC-MS, LC-FTIR, and other "coupled" analytical techniques.

3 posts Page 1 of 1
Post a reply

Column wash

avatar
Smk
Hi all
What is the best way to clean c8 column contaminant with dichloromethane, knowing that mobile phase is 95/5 H2O/ACN

Re: Column wash

avatar
HPLC chemist
Wash with 100% ACN at least 10 column volumes. Then switch to DCM since ACN is soluble in DCM.

Why are you using DCM on a C8 non-polar column?

Re: Column wash

avatar
Smk
The extracted samples some times contaminant with DCM
Post a reply
3 posts Page 1 of 1
Return to “LC-MS, GC-MS, and other”

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 11462 on Mon Dec 08, 2025 9:32 pm
Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

    Gas Chromatography

      Mass Spectrometry