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Column wash

http://www.chromforum.org/viewtopic.php?t=42091

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Column wash

Posted: Wed Mar 29, 2017 5:29 pm
by Smk
Hi all
What is the best way to clean c8 column contaminant with dichloromethane, knowing that mobile phase is 95/5 H2O/ACN

Re: Column wash

Posted: Wed Mar 29, 2017 8:05 pm
by HPLC chemist
Wash with 100% ACN at least 10 column volumes. Then switch to DCM since ACN is soluble in DCM.

Why are you using DCM on a C8 non-polar column?

Re: Column wash

Posted: Thu Mar 30, 2017 4:51 pm
by Smk
The extracted samples some times contaminant with DCM
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