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Quantitation/Qualifier Ions
Posted: Wed Jun 07, 2006 9:02 pm
by munkietoes
Are qualifier ions neccessary for quantitation of known compounds? I have an LC-MS method where I've seperated my two known compounds but when I look at the mass spec, I only have one m/z peak for each chromatographic peak. Is this desireable?
Posted: Thu Jun 08, 2006 3:50 pm
by Ary
Since you have separated your two compounds and presumably the ions you are seeing in each case are the molecular ions for each compound then yes this is indeed a desirable scenario. By having just one ion you are maximising your signal to noise ratio.
Qualifier ions
Posted: Fri Jun 09, 2006 12:16 am
by Mary Carson
You may not need qualifier ions for known compounds where you KNOW the compound is in the sample (e.g., PK studies). However, if you are doing contaminant analysis where you are trying to establish that your known compound is in an unknown sample, you probably need some qualifying ions.
Posted: Wed Jun 14, 2006 2:30 pm
by munkietoes
Thank you very much for your input!