Chromatography Forum

Hello:


Can someone recommend a good textbook for Basic HPLC?


Thanks.

I'm partial to this reference book I use when I need to check up on the "basics".

"High Performance Liquid Chromatography: Fundamental Principles and Practice"

Edited by W.J Lough

Copyright 1996

I recommend this for the basic theory, but I would keep in mind that this is a decade old. HPLC has definitely come a long way since then for applications aspects, etc.

Best Regards,
Alice

There is an awful lot of free information on the Internet, as most instrument manufacturers have information available, as well as some educational institutions providing course notes. Google can be your teacher aid.

My opinions, some of the experts here may have better suggestions.

Make sure you obtain some free catalogs - Phenomenex 2005/2006 "Chromatography Columns and Supplies" is excellent.

Merck's 2006/2007 Chrombook ( or Chromcircle CD which also has applications ), is very good.

Agilent's 2005/2006 green book " The Essential Chromatography Catalg from Agilent " ( also has GC etc ), Waters 2004/2005 "Chromatography Columns and Supplies" catalogs are good.

However if you want just one book, I would go for " Practical HPLC Method Devlopment " by Lloyd R.Snyder, Joseph J. Kirkland, Joseph L. Glajch. Second edition 1997 John Wiley ISBN 0-471-00703-X.

The technology is changing, but the fundamentals are there, as well as a good index that helps novices find their way around. It's a book that you can grow into, as it provides plenty of practical advice.

Please keep having fun,

Bruce Hamilton

There is a fairly thorough listing of text and on-line resources (if I do say so myself ) in the FAQ section. Click on the "FAQ" link near the upper left of the screen and drill down to "Where to obtain additional information"

As an introductory test, I recommend Veronika Meyer's "Practical High Performance Liquid Chromatography" (Wiley). For columns and more advanced knowledge, I recommend my book "HPLC Columns" (Wiley, VCH). A very comprehensive overview of chromatography is in Colin Poole's "The Essence of Chromatography" (Elsevier) with more references than you can read in a lifetime.

There are also some reasonable books around that specialize in applications in a particular industry, such as the Ahuja/Dong "Handbook of Pharmaceutical Analysis by HPLC" from Elsevier.

I just got a new book today which I can definitely recommend as the best beginner's book: "Modern HPLC for Practicing Scientists" by Michael W. Dong, Wiley. It is in paperback format (I did not check the price, but it should be inexpensive). It is written with the beginner in mind, with many tables and diagrams and just enough words to explain what is going on. I went through it (270 pages) in about 4 hours. Many references for more advanced reading.

Michael Dong has given many HPLC courses, and I think that his book hits the nail on the head for a beginner.

PS.: It does not hit the nail into the head of the beginner.... Just joking....

I would confer with Uwe on this one, V. Meyers text is very good for beginners. For HPLC columns in particular then Uwe's book will also be useful.

Finally for a hands on practical approach to explaining HPLC then Marvin McMaster's book: http://www.amazon.co.uk/exec/obidos/ASI ... 28-5133214 is also worthy as a easily readable reference book.

Once you get going the following book can be very helpful, especially in preventing "basic" mistakes: JW Dolan, LR Snyder, Troubleshooting LC systems, Humana Press.