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- Posts: 3
- Joined: Fri Feb 10, 2017 12:55 am
I have a method to analyze Phenylepthrine using HPLC based of the method here:
http://www.phenomenex.com/Application/Detail/3246
The only difference is I'm using a slightly lower flow rate at 0.7mL/min
It works wonderfully for Phenylephrine by itself,but now I am trying to analyze a solution with Phenelyephrine HCl, sodium chloride, sodium bisulfite, and edetate disodium and I have two peaks that are very close to each other.
Any tips on how to get these two peaks to separate better without drastically changing the method? I've already tried altering the flow rate and temperature...
Thanks!