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peak purity by Refracive index detector in HPLC
Posted: Tue May 23, 2006 4:13 pm
by jchacko
How do we determine the peak purity for stability indicatiing studies for a component that detected only by RI detector by HPLC??
Posted: Tue May 23, 2006 5:00 pm
by tom jupille
You can't. Any type of "peak purity" requires a "sample specific" detector (one that responds to some property of the analyte). RI is a "bulk property" detector; it measures a property of the solvent which is altered by the presence of analyte.
Posted: Wed May 24, 2006 4:39 pm
by Rob Burgess
If you have access to a LC-MS system you might be able to do peak purity on your RID detected component (assuming you know the mass of your analyte!)
Re: peak purity by Refracive index detector in HPLC
Posted: Mon May 29, 2006 5:08 am
by yogesh#14
How do we determine the peak purity for stability indicatiing studies for a component that detected only by RI detector by HPLC??
Hi,
The RI detector does not have a SCAN feature as is available for the UV detector. This is because the RI detector functions on a different principle altogether. The only possibility of checking wheteher the method is stability indicating is that to attach a MS to the outlet of LC and check for any impurities or any coelutions..
Hope this should help you..
Regards,
Yogesh
Posted: Mon May 29, 2006 6:34 am
by HW Mueller
Or, if jchacko doesn´t have access to other machines: Set up a considerably different chromatography run the samples with the two methods, etc.