Chromatography Forum

Dear all,
I analyze a drug which contain the latanaprost molecule using GC and have a great difficulty to identify this molecule because I did not find that topic in literature.
I used column DB-1701,30mx0,25mmx0,25 µm.
Oven temp. 80°C, hold 1 min; 30°C/min, 160°C, 10°C/min, 280°C, hold 20min.
Inj: 280°C
Det.: 280°C
Flow : 1ml/min He
Split 1/10
Solvent: 2- propanol
Ref. solution: 1 mg Latanoprost/1ml 2- propanol
Latanoprost showed up for the first time at 17 min (5 Injections). But for second sequence Latanoprost disappeared.
Was it pure coincidence? Any suggestions?

If you want to analyze anything using GC, one rather fundamental requirement is that the molecule is present as a gas at the temperatures you're using.

Have you considered this for your analysis?

Also, what kind of detection do you use?

If you want to analyze anything using GC, one rather fundamental requirement is that the molecule is present as a gas at the temperatures you're using.

Have you considered this for your analysis?

Also, what kind of detection do you use?

Looked it up here http://www.lookchem.com/Latanoprost/

Looks like a boiling point of 583.8C, unless it has a high vapor pressure below the boiling point it is probably going to be difficult to do by GC.

Thanks for the hints. I have mixed up the flash point and the boiling point.

Thanks for the hints. I have mixed up the flash point and the boiling point.

It looks much better suited for liquid chromatography