Latanoprost

[tinsang]

Dear all,
I analyze a drug which contain the latanaprost molecule using GC and have a great difficulty to identify this molecule because I did not find that topic in literature.
I used column DB-1701,30mx0,25mmx0,25 µm.
Oven temp. 80°C, hold 1 min; 30°C/min, 160°C, 10°C/min, 280°C, hold 20min.
Inj: 280°C
Det.: 280°C
Flow : 1ml/min He
Split 1/10
Solvent: 2- propanol
Ref. solution: 1 mg Latanoprost/1ml 2- propanol
Latanoprost showed up for the first time at 17 min (5 Injections). But for second sequence Latanoprost disappeared.
Was it pure coincidence? Any suggestions?

[Rndirk]

If you want to analyze anything using GC, one rather fundamental requirement is that the molecule is present as a gas at the temperatures you're using.

Have you considered this for your analysis?

Also, what kind of detection do you use?

[James_Ball]

If you want to analyze anything using GC, one rather fundamental requirement is that the molecule is present as a gas at the temperatures you're using.

Have you considered this for your analysis?

Also, what kind of detection do you use?

Looked it up here http://www.lookchem.com/Latanoprost/

Looks like a boiling point of 583.8C, unless it has a high vapor pressure below the boiling point it is probably going to be difficult to do by GC.

[tinsang]

Thanks for the hints. I have mixed up the flash point and the boiling point.

[Rndirk]

Thanks for the hints. I have mixed up the flash point and the boiling point.

It looks much better suited for liquid chromatography