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c8 column conditioning

Posted: Wed May 10, 2006 12:17 pm
by vestelshirley
I have an old zorbax c8 column and need some pointers on how to try and reclaim it.

Posted: Wed May 10, 2006 6:28 pm
by Mark Tracy
An old, unused column? Or an old column of unknown history? Either way, wash it with 50% acetonitrile for 10 min, then 100% acetonitrile for another 20 min, then test it. If it fails, don't waste any more time.