THF and Retention

[Douglas Moore]

All of us are aware that the addition of THF to the mobile phase can change the retention order of peaks. Can someone provide an reference explaining the actual mechanism that occurs to create this effect? Is there a way to predict if adding THF to the mobile phase will change retention order for any particular application?

Thanks,
Douglas Moore

[Uwe Neue]

I think that there are a few rules of thumb that are useful for a straight comparison of the selectivity of the different solvents. I can send you a poster on this from HPLC 2005. The comparison was dome between staight methanol, acetonitrile and THF.

I have not tested, how well the rules found for THF apply to mixtures of other solvents with THF, but my experience with acetonitrile - methanol mixtures is that one can interpolate reasonably well.

[ntruong]

I can send you a poster on this from HPLC 2005

Hi Uwe Neue,
Can you send me one as well? My email address is ngoc.truong@sbcglobal.net
Thanks in advance,

[asouliot]

Dear Uwe Neue,

Please send me this poster from HPLC 2005 it might be very useful for me


Thank you in advance

[ziyads]

can send you a poster on this from HPLC 2005
Hi Uwe Neue:
Can send Can you send me one a copy of this poster to the following e-mail:
ziyadchem@yahoo.com
I will be very thankfull