Carbohydrate Detection Limits [August 7, 2004]
Posted: Sun Aug 29, 2004 6:29 pm
By Brent on Saturday, August 7, 2004 - 12:35 pm:
I was trying to find a general review of HPLC monosaccharide analysis, specifially which methods have the lowest detection limits for a typical sugar as glucose. I have an LC/APCI-MS with detection limits in the hundred picogram range, and wanted to compare to fluorescence, amperometric, and GC-MS methods. Can anyone help with that info?
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By Chris Pohl on Saturday, August 21, 2004 - 05:58 pm:
Brent,
I can't speak to the other methods you mentioned but a properly operating amperometric system can routinely yield detection limits in the mid-femtomole range (i.e. 300-100 femtomoles injected on a 4 mm column).
I was trying to find a general review of HPLC monosaccharide analysis, specifially which methods have the lowest detection limits for a typical sugar as glucose. I have an LC/APCI-MS with detection limits in the hundred picogram range, and wanted to compare to fluorescence, amperometric, and GC-MS methods. Can anyone help with that info?
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By Chris Pohl on Saturday, August 21, 2004 - 05:58 pm:
Brent,
I can't speak to the other methods you mentioned but a properly operating amperometric system can routinely yield detection limits in the mid-femtomole range (i.e. 300-100 femtomoles injected on a 4 mm column).