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hydrolysis and aminoacid composition

http://www.chromforum.org/viewtopic.php?t=38811

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hydrolysis and aminoacid composition

Posted: Wed Jan 04, 2017 7:05 pm
by hplcnewuser1
Hi everyone, my product is a peptide 14aa long and I need to hydrolyze and show aminoacid content. Does anyone have an idea how to go about itas far as sample prep and HPLC method for aminoacid separation.
Any help is appreciated.

Re: hydrolysis and aminoacid composition

Posted: Mon Jan 09, 2017 11:58 pm
by tom jupille
Pretty much "cookbook" procedures. Check this link:
http://www.sigmaaldrich.com/analytical- ... lysis.html

Re: hydrolysis and aminoacid composition

Posted: Thu Jan 26, 2017 12:37 am
by mattmullaney
Here's a link to a review article of protein hydrolysis methods:

http://www.sciencedirect.com/science/ar ... 7398007213

Journal of Chromatography A, Volume 826, Issue 2, 27 November 1998, Pages 109–134, "Hydrolysis and amino acid composition analysis of proteins", by Michael Fountoulakis and Hans-Werner Lahm.
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