Area response as a function of flow

[Mike H.]

I'm working on a new RP-LC method and noticed I'm getting different area responses at different flow rates? I'm actually seeing a 25% reduction in area when going from 1.5 ml/min to 2 ml/min. Why?

I can give other parameters is required, but it doesn't seem necessary.

[tom jupille]

Area is a function of the analyte absorbance and its residence time in the flow cell.

[JI2002]

To elaborate, peak area equals peak width times absorbance. Absorbance does not change when increasing flow rate from 1.5 ml/min to 2 ml/min, however the peak width decrease by a factor of 25% (1/1.5 - 1/2)*100/(1/1.5) =25%.

[Mike H.]

Ok that was very helpful. Thank you.