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NCE research

http://www.chromforum.org/viewtopic.php?t=3865

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NCE research

Posted: Mon Apr 24, 2006 4:23 am
by yogesh#14
Hi all,

I have molecule in Preformulation study and is a form of carbohydrate.

We are expeceted to carry out solubility studies , HPLC method development . Since it is sugar molecule its difficult to detect on LC-UV (i've tried 1000PPM and 30µL) injection but response is low . Should i go for derivatisation or is it advisable to develop method on GC.

Request you kindly post your comments.

Best regards,

Yogesh

Posted: Mon Apr 24, 2006 4:46 pm
by DR
Do you have access to either a Refractive Index (RI) or Light Scattering (ELSD) detector? They both work well for carbohydrates - at least for assay once you work out suitable (and generally isocratic) conditions.
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