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Area response as a function of flow
Posted: Thu Dec 15, 2016 7:20 pm
by Mike H.
I'm working on a new RP-LC method and noticed I'm getting different area responses at different flow rates? I'm actually seeing a 25% reduction in area when going from 1.5 ml/min to 2 ml/min. Why?
I can give other parameters is required, but it doesn't seem necessary.
Re: Area response as a function of flow
Posted: Fri Dec 16, 2016 5:35 am
by tom jupille
Area is a function of the analyte absorbance and its residence time in the flow cell.
Re: Area response as a function of flow
Posted: Fri Dec 16, 2016 7:44 pm
by JI2002
To elaborate, peak area equals peak width times absorbance. Absorbance does not change when increasing flow rate from 1.5 ml/min to 2 ml/min, however the peak width decrease by a factor of 25% (1/1.5 - 1/2)*100/(1/1.5) =25%.
Re: Area response as a function of flow
Posted: Tue Jan 03, 2017 7:50 pm
by Mike H.
Ok that was very helpful. Thank you.