L-Phenylalanine extraction from a solid matrix
Posted: Mon Dec 12, 2016 2:54 pm
Hi everyone,
I'm working with solid-state fermentation of agricultural residues (like sugarcane bagasse) and I need to quantify the L-phenylalanine content in those residues. I'm able to set up and optimize a HPLC method to do so, but I'm not such confident with the sample preparation step. What I decided to do was a solid-liquid extraction using water as solvent and a further filtration through a 0.45um membrane. Have any of you deal with a similar matrix? The point is that with this simple extraction, some interferences seems to be present very close to the elution point for L-Phe and I'm not sure about the recovery of the process.
Any suggestion would be very helpful
Thanks,
I'm working with solid-state fermentation of agricultural residues (like sugarcane bagasse) and I need to quantify the L-phenylalanine content in those residues. I'm able to set up and optimize a HPLC method to do so, but I'm not such confident with the sample preparation step. What I decided to do was a solid-liquid extraction using water as solvent and a further filtration through a 0.45um membrane. Have any of you deal with a similar matrix? The point is that with this simple extraction, some interferences seems to be present very close to the elution point for L-Phe and I'm not sure about the recovery of the process.
Any suggestion would be very helpful
Thanks,