Chromatography Forum

Hi,

Is it possible to conclude that substance is present in the sample even if the peaks are not well-define and there is overlapping present in the chromatograph but the retention time is approximately the same.

If not, how to i improve the peaks if according to literature, the sample is indeed present.

Thanks.

The answers to your questions are mostly answered in this forum under "Assessing an HPLC method for determination of impurities".

thanks =)