Cysteine and Cystine [August 3, 2004]
Posted: Sun Aug 29, 2004 6:17 pm
By sc on Tuesday, August 3, 2004 - 08:48 am:
I haven't had to much luck developing a decent RPLC method for cysteine and cystine. Both compounds "blow off" of the column. Have tried many different MP combos, columns (C8, C18, amine, etc) w/ different porosities and lengths.
Any thoughts??
sc
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By Zelechonok on Tuesday, August 3, 2004 - 09:27 am:
Check this method.
http://allsep.com/makeChr.php?chr=Chr_015
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By al on Tuesday, August 10, 2004 - 02:57 pm:
you could also try this.
http://www1.dionex.com/en-us/by_techniq ... p5010.html
I haven't had to much luck developing a decent RPLC method for cysteine and cystine. Both compounds "blow off" of the column. Have tried many different MP combos, columns (C8, C18, amine, etc) w/ different porosities and lengths.
Any thoughts??
sc
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By Zelechonok on Tuesday, August 3, 2004 - 09:27 am:
Check this method.
http://allsep.com/makeChr.php?chr=Chr_015
-------------------------------------------------------------------------------------------------------
By al on Tuesday, August 10, 2004 - 02:57 pm:
you could also try this.
http://www1.dionex.com/en-us/by_techniq ... p5010.html