penicillin V in feeds HPLC
Posted: Tue Apr 04, 2006 10:07 am
I need a HPLC method for penicillin V determintion in feeds[/b]
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penicillin V in feeds HPLC
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Posted: Tue Apr 04, 2006 4:58 pm
Library!
Penicillin in Feeds
Posted: Tue Apr 04, 2006 9:42 pm
Don't be too hard on him, Mark. There's not that many published feed methods, and none (that I know of) for Pen V.
That said, try JOURNAL OF AOAC INTERNATIONAL VOL. 88, NO. 3, 2005. It's Pen G, but it would probably work for V as well.
That said, try JOURNAL OF AOAC INTERNATIONAL VOL. 88, NO. 3, 2005. It's Pen G, but it would probably work for V as well.
Posted: Wed Apr 05, 2006 3:23 am
Try this :
http://www.ncbi.nlm.nih.gov/entrez/quer ... id=1639899
This link leads to Entrez-PubMed. The abstracts mentioned there describe HPLC methods and extraction protocols for penicillin V in tissue samples. I guess you'll have to evolve your own method from them. Shouldn't be too difficult, I think. Pen V levels in feed would be higher than in tissue, and perhaps you can successfully adapt an extraction method used for Pen V in tissue samples.
Normally, I would have agreed with Mark, but Pen V in feed is not a widely published analysis.
Good luck.