Chromatography Forum

Chromatography Forum is a public discussion group where you can post questions, news, or messages of interest to chromatographers everywhere, but you must be registered to participate (registration is FREE). If you are a registered user, please log in.
http://www.chromforum.org/

problem with nereistoxin thiosultap

http://www.chromforum.org/viewtopic.php?t=3723

Page 1 of 1

problem with nereistoxin thiosultap

Posted: Thu Mar 30, 2006 5:45 pm
by aflores
I have a problem with thiosultap. I am trying analyzed it with mobil phase acn:h20(+1%H3p04) 80:20 for hplc Uv, but the spectrum is modified with the concentration. ¿Why?, and so I am not analyze. ¿anyone know a diferent mobil phase¿

Posted: Fri Mar 31, 2006 7:40 pm
by tom jupille
If the spectra are concentration-dependent, one workaround is to look at the spectra for an "isosbestic point" (essentially a "pivot" wavelength around which the absorbance bands shift) and work at that wavelength.
All times are UTC
Page 1 of 1
Powered by phpBB® Forum Software © phpBB Limited
https://www.phpbb.com/