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Reverse phase column

http://www.chromforum.org/viewtopic.php?t=3699

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Reverse phase column

Posted: Tue Mar 28, 2006 10:54 pm
by lsolines
Hi there. I'm taking a class on protein separation. My question is: if we glycosylate a peptide, would it decrease or increase the retention time in a reverse phase column?. Thanks for your time.

LSL

Reverse phase column

Posted: Wed Mar 29, 2006 1:49 am
by Chris Pohl
lsolines,

Carbohydrates are generally significantly more polar than peptides so a glycosylated peptide should also be more polar. Increased polarity will reduce retention on a reversed phase column.
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