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Sodium Perchlorate in Mobile Phase

http://www.chromforum.org/viewtopic.php?t=3661

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Sodium Perchlorate in Mobile Phase

Posted: Tue Mar 21, 2006 1:07 pm
by Chris
I am looking at the Pharmacopoeia methods for the analysis of Tylosin and they all contain Sodium Perchlorate in the mobile phase (aqueous solution with Acetonitrile at pH 2.5). Due to the risks associated with this material I would like to swap it for something else. What role does the Sodium Perchlorate play in mobile phases, is it simply a buffer that can be exchanged for something else?

Posted: Tue Mar 21, 2006 4:46 pm
by Mark Tracy
Perchlorate is an ion-pairing agent, about as strong as trifluoroacetate. NaClO4 is a neutral salt that does not buffer. Its main virtue is UV transparency. You can try a short-chain sodium alkanesulfonate as a substitute.
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