Chromatography Forum

Help! With my new job, I was introduced to Millenium23 about 3 weeks ago. The difference in processing a normal LC data and a PDA is that, in processing PDA data one has to open a method set in order to extract the chromatogramms generated to a specific wavelenght, right? Thereafter, further processing is the same as the normal HPLC data. The weight/weight assay gave 4 different results for standard A (standard agreement) using 3 different calibration curves. Two of the calibration curves used were not found in the printout. I have no idea where these calibration curves were taken. Similar results were obtained for the samples. It seems that calibration curves from previous processing had been used, although "clear calibration" had been enabled prior to processing. Any idea why this is?

It looks like you process your data manually.
I'm not sure what you mean with "clear calibration is enabled".
When you put clear calibration in your sample set, but don't enable process data, this line is useless.

When you go to review, open your method set, edit > clear calibration.
Then click apply method set. Now check in the calibration curve window if there is only 1 standard appearing. Now go further with your calibration, until the last standard. Check again if everything is alright.

Now process your sample, but check again the reference curve.

Normally this should work, otherwise let us now

Good luck

If you open your chromatogram in review window and extract requried wavelength, and then set up the processing method as normal.

If you then open your method set from the project.

Set Instruemnt method at top as normal.
Leave the processing and reporting boxes blank and instead..
Select the derive channel option and set a single wavelength at the wavelength that you wish to integrate.
You can then use this derived channel in the table at the bottom of the method set and include processing and reporting methods in the table also as required.

Then process the data using the updated method set.

It should extract the required wavelength and process as wanted (and there shoudl be but one chromatogram).