uv detection at 195 nm

[zompa]

Sorry for bothering you my friends and you tom...
I need to know your opinion and advice about detecting a substance at 195nm. The product I'm dealing with is Ibandronate (there are a couple of posts if you are interested in the matter) and the mobile phase is highly acqueous... Do I need any particular device to succeed in the detection?
Thanks to anyone who takes the bother to reply.

Zompa.

[Rafael Chust]

The main problem you will face is baseline noise ("everything" absorbs at 195 nm!) and you must be careful with your eluent (low-UV solvents, truly deionized and organic-free water, pure buffers, etc.), to avoid adding more noise to your chromatogram.

Besides that, no problemo!

[DR]

Some like to apply a nitrogen sparge to their optical bench at such wavelengths as there's stuff in the air that absorbs a bit at these wavelengths. Personally, I liken it to buying shakti stones for your stereo...

[josebenjamin]

DEar Friend,

I would like to add to the other messages with this recommendation: make sure you premix your mobile phase. All UV detectors are somewhat sensitive to refraction index changes, and even though modern pumps are quite good at on-line mixing, this problem is more severe at low wavelengths.

Also, it sounds like you would like to make sure your detector cell is very clean and your deuterium lamp is in excellent conditions (like new)

Good Luck,

josebenjamin

[zompa]

thanks to all of you...
I really appreciate your advice.

[Rafael Chust]

Dear Zompa:

I am unable to find your "ibandronate" post... is this one?