multi-vitamins detemination by HPLC?

[Crystal]

I am doing a mini project to determine vitamins A(b-carotene), B1(Thiamine), B2(riboflavin), B6(pyridoxine), C(ascorbic acid) and E(Alpha-tocopherol) in multi-vitamins tablet by HPLC with UV detector. Only C18 colunm is provided by school.

However, no HPLC method is suggested by USP, BP. So I want to know more information about mobile phase, extraction to analyze them for HPLC. Any system is good for separate these water and fat soluble vitamins?

[tom jupille]

Search the web sites of the column suppliers (e.g., agilent, waters, supelco, alltech, etc.). You should be able to find lots of applications notes to get you started.

[Bryan Evans]

See separation below:

http://www.silvertonesciences.com/modul ... TI122E.pdf

[rkrispies]

Check out:

J. Chrom. A v.1070, p 49.

Chris