Chromatography Forum

Anybody know of a wavelength accuracy check to check 205nm range of an Agilent detector? I know about the holmium oxide check but that doesnt go as low as we want. need somthing similar but in the 205 range. Thanks.

can you say why you need to perform this testing?
why can't you use Agilent's OP/PQ testing for wavelenght accuracy check?

Dear Friend,

I doubt very much that you are going to find a specific test for accuracy at 205nm. I worked on these LC aspects sometime ago and you may want to check the following reference,

B. Esquivel, "Wavelength Accuracy Testing of HPLC Detectors", Chromatographia, Vol. 26 (1988), pages 321-323.

In that work I described a Terbium ion solution in propanol that has a well defined maximum at 218.6nm (I may be slightly wrong about the value). At the time this test was introduced, another test solution of Er ion in water was alaso developed that has become somewhat popular. Waters used to sell the solution for their detectors. Unfortunately the Erbium solution only works at 254.6nm and higher values.

In my experience, everytime I test the accuracy at 218 or 254nm, the detectors where equally accurate. Therefore is loikley that your instruments will be equally good at 218 and 205nm.

Good luck,

josebenjamin

Dear Zeeknollie,

Now I also remeber that sometime ago in LC/GC there was a report of a portable diode array very carefulkly calibrated thyat could be used for accuracy test. Unfortunately I do not remeber the specific reference. For sure you could go to the website and do a search.

Good Luck,

josebenjamin