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method for phenoxy methyl penicillin in human plasma by hplc

Posted: Fri Mar 03, 2006 11:08 am
by anilray1155
i have done more than 100 method but no good result was found
here the problem was specificity
currently i try precipitation method +solvent washing
but here the problem was same and in some method the ISTD (internal standard )peak was splited
so suggest the proper solvent or solvent mixture for washing

Posted: Fri Mar 03, 2006 1:33 pm
by Uwe Neue
Your main problem is the sample preparation method. Try solid phase extraction. Good methods can be found on the Waters website. Look for the Oasis applications notebook.