I am in need of a collagenase HPLC assay with UV detection. I have a method for UV/VIS, but my concentations are too low (0.006 mg/mL) to generate a decent Absorbance reading (they are <0.1). I am not finding a lot in literature searches, and would like to know if anyone has a decent starting method (column, mobile phase, wavelength, etc.) Any assistance would be appreciated.