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Area / RT
Posted: Tue Sep 06, 2016 10:12 am
by crimelist
You know if there is a mathematical relationship between the retention time and peak area?
or they are unrelated?
Thks
Re: Area / RT
Posted: Tue Sep 06, 2016 2:34 pm
by DR
They are, for the most part, unrelated.
Area is a function of the extinction coefficient of the analyte (assuming we're talking about UV/VIS detection), its concentration and the wavelength(s) monitored.
Retention time is a function of the resolution equation which takes partition coefficient(s) and physical parameters (column length, packing properties like size and pore size, and column diameter) into account.
Have you looked into the tutorials linked among this forum's sticky posts?
If not, please do so as they do a better job of covering this stuff than i do.
Re: Area / RT
Posted: Tue Sep 06, 2016 2:43 pm
by Consumer Products Guy
Yes, area of the peak can vary as the retention time varies when HPLC conditions are changed.
In my job there were so many projects "in queue" that I never needed or wanted to learn why this was so, just was important to know that it can vary as conditions are changed.
Re: Area / RT
Posted: Tue Sep 06, 2016 5:17 pm
by GOM
Nice to hear from CPG and DR (I have missed your Felix the Cat icon posts - always made me smile
)
In answer to your query
as posed
You know if there is a mathematical relationship between the retention time and peak area?
or they are unrelated?
There is no mathematical relationship between analyses under the same conditions- think about it
Unless I am missing something
Regards
Ralph