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Column wash

Discussions about GC-MS, LC-MS, LC-FTIR, and other "coupled" analytical techniques.

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Column wash

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Smk
Hi all
What is the best way to clean c8 column contaminant with dichloromethane, knowing that mobile phase is 95/5 H2O/ACN

Re: Column wash

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HPLC chemist
Wash with 100% ACN at least 10 column volumes. Then switch to DCM since ACN is soluble in DCM.

Why are you using DCM on a C8 non-polar column?

Re: Column wash

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Smk
The extracted samples some times contaminant with DCM
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