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N,N-Diethyl-meta-toluamide (DEET) in cream repellent

Posted: Sun Aug 28, 2016 3:03 pm
by Fran Chiara
Hello! Can anyone tell me the best way to extract and quantify DEET in any cream repellent please?

I allready thought to use methanol as solvent, a C18 column and a UV detection at 220nm.

Thanks for your help!

Re: N,N-Diethyl-meta-toluamide (DEET) in cream repellent

Posted: Mon Aug 29, 2016 12:32 am
by Consumer Products Guy
Fran - our company never had an insect repellant product, so I was never asked to assay for DEET.

However, I typically do not like HPLC procedures with low absorbance wavelengths like 220nm, especially for products such as these that contain DEET at relatively high levels. I searched DEET structure, it has an aromatic ring, likely has absorbance higher than 250nm which would give much better selectivity compared to product matrix components.

If me, I'd dissolve pure DEET in solvent, and run UV scan (or even HPLC DAD scan) and pick a higher wavelength for better selectivity. I see online a procedure using 254nm, that sounds much better, but whenever I see "254nm" I wonder if someone had only an outdated single-wavelength UV detector available!!! http://www.bmsw.net.cn/EN/abstract/abstract10604.shtml

To extract from a cream base, I would try DMF and I would try methanol as first choices, but first try DEET in those to check on its solubility. If DEET dissolves in a solvent, and the finished product dissolves readily or disperses readily in that same solvent, you're most of the way there. Keep injection volume small (5 microliters or less) if injecting that organic solvent extract into a mobile phase high in aqueous.

Start with RP-18 and try to keep the procedure isocratic for simplicity if you can. My guess is that this assay should not be too challenging.

Re: N,N-Diethyl-meta-toluamide (DEET) in cream repellent

Posted: Mon Aug 29, 2016 6:04 am
by Peter Apps
Hello Fran

Welcome to the forum.

DEET can be done by GC, and if you have the hardware that might be a better option.

Peter

Re: N,N-Diethyl-meta-toluamide (DEET) in cream repellent

Posted: Mon Aug 29, 2016 2:39 pm
by Fran Chiara
Thanks you guys!