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Analytical HPLC column comparison
Posted: Mon Feb 20, 2006 8:17 am
by amirchem
Hallo
Where can I find a good RP column comparison? I am especially interested in comparison of characteristics of stationery phases from Merck, Agilent and Phenomenex.
Best regards
Posted: Mon Feb 20, 2006 9:13 am
by bartjoosen
Posted: Mon Feb 20, 2006 6:16 pm
by Uwe Neue
Column selector
Posted: Tue Feb 21, 2006 8:34 am
by Mach
Posted: Tue Feb 21, 2006 3:30 pm
by DR
Phenomenex's web site features a cross reference guide that will clue you in as to the different carbon loads, surface areas etc. for different columns too. (at least it used to, I haven't been there in a while).
Posted: Wed Feb 22, 2006 9:29 am
by Rafael Chust
I would go with Mach and DR hints...
Posted: Wed Feb 22, 2006 7:36 pm
by tom jupille
The USP has two different column selectivity databases (different ways of measuring) up on their web site. I believe this is a beta-test:
http://www.usp.org/USPNF/columnsDB.html
Posted: Thu Feb 23, 2006 1:01 am
by syx
Could you please explain the abbreviations found in the USP website? I could not find the description about them in there…
USP Database: F - Hy - CTF - CFA - TFA - BD
PQRI Database: H - S - A - B - C(2.
- C(7.0)
Posted: Thu Feb 23, 2006 2:59 am
by Uwe Neue
The webside is still growing, and such things as explanations of the stuff need to be added, as well as more data on the USP data base, and a few other things...
In both cases, the F-factor is a matching factor between the reference column and the closest columns in the data bank.
For the USP data, HY describes the hydrophobicity of the packing, CTF is the tailing factor for a chelator, CFA is the capacity factor of amitriptyline, a basic compound, TFA is the tailing factor of amitriptyline, and BD is the bonding density of the packing.
For the PQRI data base, you have a parameter for the Hydrophobicity of the packing, one for the Steric selectivity, one for hydrogen bond Acidity, one for hydrogen bond Basicity, and C for ion-exChange at pH 2.5 and pH 7.0.