Chromatography Forum

I am doing a mini project to determine vitamins in multi-vitamins tablet by HPLC with UV detector. Only C18 colunm is provided.

I would like to know any system is good for separating vitamins A(b-carotenea) and E(Alpha-tocopherol) at the same time? And the extraction method if the tablet can not be dissolved in the mobile phase completely?Thank you!

Go to the Waters.com website. Find the Waters Chemistry eApplications Notebook. Select V for Vitamins and then select the application for the Atlantis column (720000472EN.106). It covers polar and hydrophobic vitamins in a single run.

Do Google search on "HPLC of vitamins" to get all references. You can also try do go to your column manufacturer site and find it there (Agilent, Phenomenex, Waters or else).

http://www.chem.agilent.com/scripts/gen ... &prodcol=N

http://www.esainc.com/applications/appl ... tivitamins

http://www.alltechweb.com/productinfo/t ... atographic Conditions

regards,

Vlad

Hi Crytal,
I hope I can help you. You can try this :

MP : Methanol : H2O (98 : 2)
WL : 280 nm
Column : Synergi 4 Hydro RP 80 A (150 x 4.6 mm 4micron), Phenomenex
Sample and standard dilute in MeOH

For the extraction, I grind the tablet very fine, and partition it between water and ethylacetate for 15 min at 60 °C, then centrifuge to separate the phases. The fat-soluble vitamins in some multivitamins are in a waxy carrier that does not dissolve well at room temperature. The water gets rid of some of the dyes and miscellaneous interferences. It hurts the peak shape some, but I just inject the filtered ethylacetate phase; evaporation and reconstitution just makes the recovery worse.

Our Cadenza column is a great choice for the separation
of fat soluble vitamins:

http://www.silvertonesciences.com/modul ... TI121E.pdf