Chromatography Forum

Posted: **Sat Feb 18, 2006 2:23 pm**

I am doing a mini project to determine vitamins A(b-carotene), B1(Thiamine), B2(riboflavin), B6(pyridoxine), C(ascorbic acid) and E(Alpha-tocopherol) in multi-vitamins tablet by HPLC with UV detector. Only C18 colunm is provided by school.

However, no HPLC method is suggested by USP, BP. So I want to know more information about mobile phase, extraction to analyze them for HPLC. Any system is good for separate these water and fat soluble vitamins?

Posted: **Sun Feb 19, 2006 6:24 am**

Search the web sites of the column suppliers (e.g., agilent, waters, supelco, alltech, etc.). You should be able to find lots of applications notes to get you started.

Posted: **Thu Feb 23, 2006 12:18 pm**

See separation below:

http://www.silvertonesciences.com/modul ... TI122E.pdf

Posted: **Tue Mar 14, 2006 9:05 pm**

Check out:

J. Chrom. A v.1070, p 49.

Chris

Chromatography Forum

multi-vitamins detemination by HPLC?

multi-vitamins detemination by HPLC?