PA1 backflushing...can column be saved?
Posted: Mon Aug 22, 2016 9:37 am
Hi,
So we run a PA1 column with water elution (1.1ml min) for sugars with post-column addition (0.3ml/min) of 300mM NaOH for electrochemical detection. Unfortunately the column pump stopped and for ~2 hours the post column pump kept going meaning that the column was backflushed. The peaks are now much less sharp and I can not resolve some sugars. Is there any way to get the column performance back? I have tried NaOH and NaOAc.
This is something that has happened before and is an expensive problem as I have had to throw the column away and buy another one. Is there any way in the software (Chromeleon 6.
to get the post-column pump to turn off if the column pump stops?
Thanks
Dan
So we run a PA1 column with water elution (1.1ml min) for sugars with post-column addition (0.3ml/min) of 300mM NaOH for electrochemical detection. Unfortunately the column pump stopped and for ~2 hours the post column pump kept going meaning that the column was backflushed. The peaks are now much less sharp and I can not resolve some sugars. Is there any way to get the column performance back? I have tried NaOH and NaOAc.
This is something that has happened before and is an expensive problem as I have had to throw the column away and buy another one. Is there any way in the software (Chromeleon 6.
to get the post-column pump to turn off if the column pump stops?Thanks
Dan